My research background has been in the development of tools for ‘genome writing’ in mammalian cells. This involved the bottom-up synthesis and site-specific delivery of large (>100kb) DNA constructs, which facilitates multiplex editing over large genomic windows and the introduction of large amounts of novel genetic information. Subsequently, I have advanced techniques for the multiplexed generation and single-cell analysis of large structural variants including extrachromosomal circular DNAs (ecDNAs) in mammalian genomes. In January 2024, I established my lab at the University of Washington and the Seattle Hub for Synthetic Biology with the support of an NIH DP5 Early Independence Award. In my research group, we focus on developing technologies at the intersection of mammalian genome engineering, large-scale (>100kb) DNA synthesis, single-cell sequencing and de novo protein design. With these tools, we aim to construct improved disease models, elucidate the mechanisms of gene regulation, to probe the limits of genome structure and to encode novel functionalities into mammalian cells for applications in biologics production, cell therapy and cultivated meat.